Freezing/Vitrification

Introduction

A new and advanced method of freezing eggs, embryos, and sperm that uses cryopreservation methods is known as vitrification. The fundamental advantage of this procedure is that it removes the water molecules and replaces them with a higher concentration of cryoprotectant which ensures that there are no ice crystals formed in the course of the freezing procedure – and thereby keeping the cells from damage for future use. The temperature change is in the order of -12,000C per minute which ensures that there is no time sufficient for the ice crystals to form and harm the embryos.

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